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Image Search Results
Journal: Experimental Biology and Medicine
Article Title: Diabetic human adipose tissue-derived mesenchymal stem cells fail to differentiate in functional adipocytes
doi: 10.1177/1535370216681552
Figure Lengend Snippet: hASC (healthy) (a) and dASC (diabetes) (b) cells after 21 days of adipogenic differentiation viewed by light microscopy. Lipid droplets accumulation measured by Oil Red staining in hASC (c) and in dASC (d) after 21 days of adipogenic differentiation. ASC: adipose stem cell. (A color version of this figure is available in the online journal.)
Article Snippet: Adipose stem cells (ASCs) culture and differentiation hASC and
Techniques: Light Microscopy, Staining
Journal: Experimental Biology and Medicine
Article Title: Diabetic human adipose tissue-derived mesenchymal stem cells fail to differentiate in functional adipocytes
doi: 10.1177/1535370216681552
Figure Lengend Snippet: Analysis of gene expression by real-time PCR of CEBPα, FAS, FABP4, DGAT1, SCD-1, SREBP-1c, and DLK-1 of hASC (healthy) and dASC (diabetes) before and after 21 days of adipogenic differentiation. All values are expressed as mean ± SEM of four experiments (n = 4) in duplicate. (#p < 0.05 versus hASC at 0 day; *p < 0.05 versus dASC at 0 day.) ASC: adipose stem cell; CEBPα: CCAAT/enhancer-binding protein alpha; DGAT1: diacylglycerol O-acyltransferase 1; DLK-1: delta like non-canonical Notch ligand 1; FAS: fatty acid synthase; FABP4: fatty acid binding protein 4; hASC: healthy adipose stem cell; PCR: polymerase chain reaction; SCD-1: stearoyl-CoA desaturase-1; SREBP-1c: sterol regulatory element-binding protein 1c.
Article Snippet: Adipose stem cells (ASCs) culture and differentiation hASC and
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Binding Assay, Polymerase Chain Reaction
Journal: Experimental Biology and Medicine
Article Title: Diabetic human adipose tissue-derived mesenchymal stem cells fail to differentiate in functional adipocytes
doi: 10.1177/1535370216681552
Figure Lengend Snippet: Analysis of gene expression by real-time PCR of cytokines IL-1β, IL-6, and TNFα of hASC (healthy) and dASC (diabetes) before and after 21 days of adipogenic differentiation. All values are expressed as mean ± SEM of four experiments (n = 4) in duplicate. (#p < 0.05 versus hASC at 0 day; *p < 0.05 versus dASC at 0 day.). ASC: adipose stem cell; IL: interleukin; PCR: polymerase chain reaction; SEM: mean standard error; TNFα: tumor necrosis factor alpha.
Article Snippet: Adipose stem cells (ASCs) culture and differentiation hASC and
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction
Journal: Experimental Biology and Medicine
Article Title: Diabetic human adipose tissue-derived mesenchymal stem cells fail to differentiate in functional adipocytes
doi: 10.1177/1535370216681552
Figure Lengend Snippet: Analysis of gene expression of PPARα, PPARδ, and PPARγ by real-time PCR of hASC (healthy) and dASC (diabetes) before and after 21 days of adipogenic differentiation. All values are expressed as mean ± SEM of four experiments (n = 4) in duplicate. (#p < 0.05 versus hASC at 0 day.). ASC: adipose stem cell; PCR: polymerase chain reaction; PPAR: peroxisome proliferator-activated receptor.
Article Snippet: Adipose stem cells (ASCs) culture and differentiation hASC and
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction
Journal: Experimental Biology and Medicine
Article Title: Diabetic human adipose tissue-derived mesenchymal stem cells fail to differentiate in functional adipocytes
doi: 10.1177/1535370216681552
Figure Lengend Snippet: Analysis of gene expression by real-time PCR of IRS-1 and SIRT1 of hASC (healthy) and dASC (diabetes) before and after 21 days of adipogenic differentiation. All values are expressed as mean ± SEM of four experiments (n = 4) in duplicate. (#p < 0.05 versus hASC at 0 day.). ASC: adipose stem cell; IRS-1: insulin receptor substrate 1; PCR: polymerase chain reaction; SIRT-1: sirtuin 1.
Article Snippet: Adipose stem cells (ASCs) culture and differentiation hASC and
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction